DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange
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چکیده
منابع مشابه
DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange
RAD51, an essential eukaryotic DNA recombinase, promotes homologous pairing and strand exchange during homologous recombination and the recombinational repair of double strand breaks. Mutations that up- or down-regulate RAD51 gene expression have been identified in several tumors, suggesting that inappropriate expression of the RAD51 activity may cause tumorigenesis. To identify chemical compou...
متن کاملHomologous pairing and DNA strand-exchange proteins.
PRINCIPLES OF HOMOLOGOUS PAIRING AND DNA STRAND EXCHANGE . Homologous Pairing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ... . . . . . . . DNA Strand Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Energetic Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Experimental Assays . . . . . . . . . . . . . . . . . ...
متن کاملHuman PSF binds to RAD51 and modulates its homologous-pairing and strand-exchange activities
RAD51, a eukaryotic recombinase, catalyzes homologous-pairing and strand-exchange reactions, which are essential steps in homologous recombination and recombinational repair of double strand breaks. On the other hand, human PSF was originally identified as a component of spliceosomes, and its multiple functions in RNA processing, transcription and DNA recombination were subsequently revealed. I...
متن کاملA chemical compound that stimulates the human homologous recombination protein RAD51.
RAD51 and other members of the RecA family of strand exchange proteins assemble on ssDNA to form presynaptic filaments, which carry out the central steps of homologous recombination. A microplate-based assay was developed for high-throughput measurement of hRAD51 filament formation on ssDNA. With this method, a 10,000 compound library was screened, leading to the identification of a small molec...
متن کاملHuman Rad51 Protein Promotes ATP-Dependent Homologous Pairing and Strand Transfer Reactions In Vitro
The human testis Rad51 protein, a structural homolog of E. coli RecA, binds single- and double-stranded DNA and exhibits DNA-dependent ATPase activity. Using circular ssDNA and linear dsDNA (3.0 kb in length), we demonstrate that hRad51 promotes homologous pairing and strand exchange reactions in vitro. Joint molecule formation was dependent upon ATP hydrolysis and DNA homology and was stimulat...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2009
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkp200